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How to clean glassware

2020-11-20 09:49:41
a

How to clean laboratory equipment

1. Washing
1. Washing of glassware
1.1 Immersion
Before using the new bottle, it should be briefly washed with tap water, and then soaked in dilute hydrochloric acid solution (5%) overnight; the cultured glassware should be immersed in clean water immediately after use; Note: let the water completely enter the bottle, and should not be left behind. There are bubbles.
1.2 Brushing
After soaking, the glassware should be washed with a brush dipped in detergent (a soft brush and a good detergent, such as high-grade washing powder or detergent) should be used, and sand-containing decontamination powder cannot be used! When washing, pay special attention to the corners of the bottle.
1.3 Immersion
The vessel should be filled with cleaning solution without leaving air bubbles. The soaking time should not be less than 6 hours; generally it should be soaked overnight.
Cleaning fluid formula:
【Strong liquid】 Potassium dichromate 63g
Concentrated sulfuric acid 1000ml
Distilled water 200L
【Secondary strength liquid】 Potassium dichromate 120g
Concentrated sulfuric acid 200ml
Distilled water 1000ml
【Weak Liquid】 Potassium Dichromate 100g
Concentrated sulfuric acid 100ml
Distilled water 100ml
Sodium silicate lotion;
It is safer to use and can replace cleaning fluid, but it is more expensive.
First prepare 100X stock solution: potassium silicate 80g, sodium metaphosphate 9g, heat and dissolve in 1000ml distilled water. When using, dilute 100 times with distilled water.
1.4 Flushing
After brushing and pickling, it needs to be rinsed thoroughly with water so that no residue is left. A washing device should be used for rinsing to ensure the rinsing effect. If manual operation is used, each bottle must be filled with water, poured out, repeated more than ten times, and then rinsed with distilled water for 2 to 3 times, and dried for later use.
2. Cleaning of rubber stopper
Rinse the newly purchased rubber stopper with tap water first, and then do the conventional treatment.
Regular washing method:
soak

2% NaOH boil (10-20 minutes)

Rinse with tap water

Soak in 1% dilute hydrochloric acid (30 minutes)

Rinse with tap water

Distilled water cleaning (2 to 3 times)

dry for use
3. Cleaning of plastic utensils
At present, the plastic utensils used for cultivation in my country are mainly purchased from abroad, which are non-toxic and sterilized and sealed and packaged. When using, just open the package, it is a one-time use item. If necessary, after aseptic treatment after use, it can be used repeatedly 2 to 3 times, but it should not be too much. Cleaning and sterilization are still required for reuse. Plastic utensils are soft in texture and should not be washed with a brush, which makes cleaning difficult. For this purpose, one is to prevent scratches, and the other is to immerse it in water immediately after use to prevent the adhesion from drying out; if there is any residual adhesion, it can be wiped off with absorbent cotton, rinsed with running water, dried, and then used 2% NaOH solution Soak overnight, fully rinse with tap water, then soak in 5% hydrochloric acid solution for 30 minutes, then rinse with tap water and distilled water, and dry it for later use.
2. Disinfection
1. Ultraviolet disinfection
Direct ultraviolet irradiation is very convenient and effective, and is currently a commonly used disinfection method in various laboratories.
[Scope of application] Air, the surface of the operating table and some culture vessels that cannot be sterilized by other methods (such as plastic culture dishes, culture plates, etc.)
[Requirements] (1) The ultraviolet lamp in the cultivation room should be 2.5 meters away from the ground, so that there is 0.06 microwatts of energy per square centimeter, so that effective disinfection can occur. (2) Due to the different sensitivities of various bacteria to ultraviolet rays, the irradiation time and dose are also different.
[Disadvantages] (1) It produces ozone, pollutes the air, and is harmful to the body; (2) The parts that cannot be irradiated by rays cannot be disinfected, so items should not be shielded from each other during disinfection.
[Note] Some people are accustomed to performing experimental operations while irradiating, which is not good. First, ultraviolet rays will have adverse effects on cells, reagents and culture medium, and second, they will also damage human skin.
2. Moist heat disinfection
Moist heat sterilization, namely high pressure steam sterilization, is an effective method.
【Scope of application】Cloths, rubber stoppers, metal instruments, glassware and some culture liquids.
【Require】
Culture liquid, rubber products 10 lbs 10 minutes
Cloth, glass products, metal equipment, etc. 15 pounds 20 minutes

3. Filtration and disinfection
【Scope of application】 Most of the culture fluids, such as synthetic culture fluid, serum, enzyme solution, etc. (these will be denatured at high temperature and lose their function and need to be sterilized by filtration.
【Commonly used filters】 Zeiss filters, microporous membrane filters and disposable filters of various specifications. Commonly used pore size 0.22um filter membrane can filter general liquid.
【Notice】
(1) The filter membrane is discarded after use, and the filter cleaning is also more convenient. First, use a brush dipped in detergent to clean it, rinse it with tap water, then rinse it with distilled water, and let it dry;
(2) Install a new filter membrane before use;
(3) Do not twist the knob too tightly during disinfection, and wrap all parts in contact with air with paper to ensure the effect of disinfection;
(4) After sterilization, tighten the knob immediately in a sterile environment;
(5) When filtering a small amount of liquid, use a small filter that can be installed on a syringe and use the same filter membrane. When filtering, put the filtrate into the syringe needle, and press out the filtrate and inject it into a sterile container.

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